【mIL 10】重組小鼠白介素-10
產(chǎn)品報(bào)價(jià)
¥800/2μg ¥2080/10μg ¥74880/1mg
背景資料
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
產(chǎn)品描述
小鼠白介素-10重組蛋白是在大腸桿菌中重組表達(dá)的,是一條單鏈、非糖基化的多肽鏈,包含有161個(gè)氨基酸,分子量為18785Da。該小鼠白介素-10重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主
大腸桿菌中重組表達(dá)
序列
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln.
溶解建議
提供的小鼠白介素-10(mIL 10)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末;該凍干粉末是從含小鼠白介素-10(mIL 10)的PBS溶液中經(jīng)進(jìn)一步透析并凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100μg/ml,后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存建議
小鼠白介素-10(mIL 10)在室溫可保存3周,或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延長(zhǎng)保存時(shí)間,建議分裝(加載體蛋白如0.1% HSA或 BSA)后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
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